None

None, but Lumpy the Clown looks down to discovers that Red the unicycle is no longer beneath him. He then falls and is not seen again for the duration of the short.

None - film is dialogue free

A rumor is that there is a middle finger, it is possibly that the clown's fingers are long

None

Despite the short being perfectly tame, some may feel emotional (and even depressed) towards the film's grim ending.